Postdoc in Protein Engineering and Spatial Glycobiology of Microbial Biofilms

Would you like to develop new fluorescent tools to visualize the sugar polymers that hold microbial biofilms together? We are

looking for a curious and collaborative postdoctoral researcher to develop a new generation of molecular probes and use them to

study complex microbial communities. At the Faculty of Engineering and Science, Department of Chemistry and Bioscience, Aalborg

University, a full-time postdoctoral position based in Aalborg, Denmark, is open for appointment from 1 February 2027 or as soon

as possible thereafter. The appointment is for 35 months, with an expected end date of 31 December 2029, and is linked to the

GlycoProbe project (Catalytically Inactive Glycanase Probes for Resolving Exopolysaccharides in Complex Microbiomes). Your work

tasks Your primary responsibility will be to develop and validate fluorescent molecular probes based on catalytically inactive

glycanases. These enzymes will be engineered to retain their selective binding to specific exopolysaccharides while losing their

catalytic activity. Your work will involve molecular cloning, recombinant protein expression and purification, structure-informed

protein engineering, site-directed mutagenesis, and fluorescent labeling. You will evaluate probe specificity and binding

properties using purified reference polysaccharides and defined microbial cultures and optimize the probes for fluorescence

imaging. In collaboration with colleagues in microbiology and bioinformatics, you will identify environmentally relevant

glycanases associated with exopolysaccharide production and degradation in complex microbial communities. This work will combine

genome-resolved microbiome analysis, enrichment cultures, and transcriptomic approaches to identify promising candidate enzymes,

which will subsequently be incorporated into the probe-development and validation pipeline. You will also apply the developed

probes to investigate the spatial organization of exopolysaccharides in activated sludge flocs, granules, and microbial biofilms.

By combining fluorescence microscopy with fluorescence in situ hybridization (FISH), you will investigate relationships between

exopolysaccharide distribution, microbial identity, and community structure. The position includes experimental planning, data

analysis, preparation of scientific publications, and collaboration with researchers at Aalborg University and Professor Jochen

Schmid at the University of Münster. Your competencies    Applicants must hold a PhD in biochemistry, structural biology,

molecular biology, biotechnology, or a closely related discipline. Demonstrated hands-on experience in molecular cloning,

recombinant protein expression and purification, and protein engineering is essential. A strong understanding of protein

structure–function relationships is particularly important. Experience with site-directed mutagenesis, carbohydrate-active

enzymes, biochemical binding assays, or fluorescent protein labeling is highly desirable. Experience in microbiology,

bioinformatics, metagenomics, and transcriptomics would be an advantage. Qualification requirements Appointment as postdoc

requires academic qualifications at PhD level. Who we are     At the Department of Chemistry and Bioscience (BIO), our

high-level research spans from basic to applied within both natural sciences and engineering. With approx. 200 employees, 75 PhD

students, and 775 bachelor and master’s students at our campuses in Aalborg (main campus) and Esbjerg, we are a vital research and

educational institution when it comes to creating new basic knowledge as well as contributing to solving the world’s challenges

within biology, chemistry, biotechnology, bioengineering, environmental science, and chemical engineering. The postdoc will join

the Microbial Ecology and Biofilms research group at the Center for Microbial Communities, led by Associate Professor Morten Kam

Dahl Dueholm. The project combines protein engineering, molecular microbiology, genome-resolved analysis, and spatial imaging,

with access to the relevant research facilities and international expertise. We continuously improve our department as an

attractive place of work and study. We offer flexible hours, social activities throughout the year both at the department level

and in each Section. We are inclusive and have respect for individuals, communities, and cultures. Our work culture is founded on

openness, curiosity, transparency, and involvement. Come join us! Read more about the department here; Welcome to the Department

of Chemistry and Bioscience - en.bio.aau.dk - Aalborg University How to apply  Your application must include the following:

• Application, stating reasons for applying, qualifications in relation to the position, and intentions and visions for the

position - Curriculum Vitae (CV) - Diplomas (master's degree diploma and PhD diploma) - List of publications, with an

indication of the attached publications that you wish to be taken into account in the assessment. You may attach a maximum of five

publications. - Documentation of teaching qualifications if relevant. - Dissemination qualifications, including participation on

committees or boards, participation in organisations etc. - Additional qualifications in relation to the position -

References/recommendations You can read more about the requirements for your application here.    The application must be

submitted via Aalborg University’s recruitment system, which can be accessed under the job advertisement on Aalborg University's

website.  Aalborg University wants to reflect the surrounding society and has diversity as a core value. Therefore, everyone,

regardless of personal background and orientation, is encouraged to apply for the position.  Aalborg University has a policy of

parallel language use. International staff are encouraged, with support from the university, to learn Danish during their

employment in order to support their integration into the workplace and Danish society. Danish language courses will be offered

as part of the employment. Do you have any questions?  If you have any questions about the position, you are more than welcome to

contact us. You will find contact persons at the bottom of the jobpost.     Further information  Read more about our recruitment

process here   The appointment process at Aalborg University involves a shortlisting process. You can read more about the

shortlisting and appointment process here.  The hiring process at Aalborg University may include a risk assessment as a tool to

identify potential risks associated with new hires, ensuring the safety, compliance, and integrity of the workplace.  Salary and

terms of employment The employment is in accordance with the Ministerial Order on the Appointment of Academic Staff at

Universities and the Ministerial Order on Job Structure for Academic Staff at Universities. Furthermore, your terms of employment

and salary are in accordance with the collective agreement between the Danish Confederation of Professional Associations and the

state (AC collective agreement) and protocol on certain terms of employment of academic staff at universities. You can find

information about the salary and salary structure for the position in the Salary Overview for Recruitment - Scientific positions

(VIP). Please refer to the introductory section of the page and the section entitled “Postdoc”.