Postdoctoral Researcher in Single-Cell Epigenetics

Do you want to contribute to top quality medical research? The Lambo research team at the department of Oncology-Pathology

(OnkPat) is an exciting new research group that studies the epigenetic mechanisms by which tumors adapt to treatment. We use

cutting edge experimental and computational methods to study epigenetics, in particular methods that utilize single cell

sequencing technologies, which includes new technologies to generate single cell DNA methylation at scale. We are an

interdisciplinary group that combines experimental perturbations, computational analysis and AI modelling with the goal to bridge

the gap between basic tumor biology and clinical implementation. As a postdoctoral researcher at our team, you have the exciting

opportunity to explore a largely uncharted field of research, to pursue your own ideas and to make the next step in your career. A

creative and inspiring environment with wide-ranging expertise and interests The Department of Oncology-Pathology is a department

where preclinical and clinical cancer research are tightly interconnected. More than 35 research groups form a dynamic and strong

research environment with a common goal to improve diagnostics, treatments, and quality of life for patients with cancer. Our

facilities are located at Bioclinicum within the Karolinska University Hospital while part of the department is located within the

Science for Life Laboratory. This provides access to advanced technologies including genomics, proteomics, metabolomics, spatial

biology, cellular and molecular imaging, and bioinformatics. Our close connection with The Swedish Childhood Tumor Biobank, The

Theranostic Trial Center, and The Karolinska Comprehensive Cancer Center, enable pre-clinical and clinical scientists to engage in

collaborative projects for early clinical testing of precision therapies, to enhance cancer diagnostics, to facilitate early

detection and to develop innovative clinical trials in cancer patients. Your mission The position involves generating, processing

and analyzing single cell methylation data for pediatric cancers, in particular pediatric acute myeloid leukemia. The postdoc will

be involved in setting up a recently developed method for generating single cell DNA methylation at scale and use the resulting

data to study pediatric tumor evolution. Because of the novelty of the data type there are opportunities to develop and publish

new computational approaches to work with this data and study tumor biology. Key duties include:

• Facilitate setting up single

cell methylation at scale - Processing and maintaining large sequencing datasets - Developing new computational approaches for

characterizing cell states and shifts in cell states based on DNA methylation - Developing new computational approaches for

identifying biologically relevant mechanisms - Working together as a team with experimental biologists to guide the generation

hypotheses, place biological findings in context and to validate findings - Performing multimodal integration using other data

modalities - Data interpretation and generating new testable hypotheses - Be responsible for study design, computational methods,

writing, data visualization, manuscript preparation, presenting and managing of data involved in publications Your profile To be

eligible for employment as a postdoctoral researcher, a PhD or a foreign degree deemed to be equivalent to a Swedish PhD is

required. This eligibility requirement must be fulfilled at the latest at the time of the employment decision. Completion of your

doctoral degree within the last three years is considered an advantage. If there are special reasons, your degree may have been

completed earlier. Qualifications and experience:

• Documented experience in analyzing single-cell, single-nucleus and/or

epigenetic datasets - Strong programming skills in R and/or Python - Experience handling large and complex biological datasets -

Experience with key analytical tasks including alignment, quality control, data integration, and biological interpretation - The

ability to develop and troubleshoot computational approaches, ensure reproducibility and critically interpret results - Documented

experience of being able to successfully disseminate research i.e. through national/ international conferences - Good

communication skills in English and the ability to work in an interdisciplinary environment Additional merits:

• Experience in

generating single cell sequencing libraries - Experience working in the wet lab - Experience working with pediatric cancer data -

Documented experience in working with/studying DNA methylation - Knowledge/experience in epigenetic regulation of developmental

biology - Documented experience in generating computational workflows i.e. published packages or bioinformatic tools Applicants

having a multidisciplinary background (both wet lab and computational experience) are prioritized for this position. Having

experience with pediatric cancer and DNA methylation is preferred but not required. What do we offer? A creative and inspiring

environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities.

At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. At KI,

you get to meet researchers working with a wide range of specialisms and methods, giving you ample opportunity to exchange

knowledge and experience with the various scientific fields within medicine and health. It is the crossover collaborations, which

have pushed KI to where it is today, at the forefront of global research. Several of the people you meet in healthcare are

educated at KI. A close relationship with the health care providers is important for creating groundbreaking top quality education

and research. Karolinska Institutet is also a state university, which entitles you to several benefits through our collective

agreement. Location: BioClinicum, Solna Choose to work at KI – Ten reasons why Application An employment application must contain

the following documents in English or Swedish:

• PhD certificate - A complete resumé, including date of the thesis defence, title

of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work - A

complete list of publications - A summary of current work (no more than one page) KI applies individual and differentiated salary

setting in accordance with our collective agreements RALS and RALS-T The application is to be submitted through the Varbi

recruitment system. At the latest 12th November. This position is for 2 years with the possibility of extension. Want to make a

difference? Join us and contribute to better health for all!