Research Assistant in single cell method development and immune regulation

Do you want to contribute to top quality medical research? Join the research group of Sophie Bensing at the Department of

Medicine, Solna, Division of Experimental Endocrinology based at the Centre for Molecular Medicine (CMM). Our group combines and

develops novel single cell genomics approaches to understand how transcriptional regulation and alternative splicing shape immune

cell states, with a particular focus on T-cell regulation, endocrine organs and autoimmune disease. We are particularly interested

in how immune cells respond immediately to stimulation and how these early molecular events may shape later cell fate and

functional outcomes. We are looking for 1-2 research assistants that will work at the interface of experimental and computational

biology, single-cell technologies and immunology in a collaborative, method-driven research environment. Your mission As a

research assistant, you will be tasked with providing experimental and practical support for ongoing projects within multi-omic

method development and time-course and pertubation focused experiments untangling how immediate response might drive transient

cell states and fates. Main responsibilities:

• Process, prepare and quality control for single-cell transcriptomics experiments

as well as RNA extraction, cell culture, according to established protocols - Assist with analysis of single cell sequencing data

• Organize experimental data, maintain accurate laboratory records and contribute to preliminary analysis - Collaborate with team

members, present progress in group meetings and support routine laboratory operations Your profile We are looking for 1-2

enthusiastic, motivated, and collaborative early-career research assistants with an interest in experimental biomedical research,

single cell methods and transcriptional regulation. You should be well organized, eager to learn and comfortable working with

biological samples and established protocols. Required qualifications:

• A Master’s degree in Biomedicine, Molecular Biology, Cell

Biology, Biotechnology, or a related field, or an equivalent degree expected to be completed before the start of employment -

Documented hands-on experience in a biological or biomedical laboratory, including relevant thesis research - Ability to follow

experimental protocols precisely and maintain structured laboratory records - Good communication skills in English, both spoken

and written - Ability to work independently on assigned tasks and collaborate effectively with colleagues Meritorious

qualifications: - Experience with one ore more of the following: RNA extraction, cell culture, FACS sorting, single cell RNA-seq,

CRISPR assays, spheroid/organoid cultures, and animal work - Familiarity with single-cell transcriptomics, sequencing sample

preparation or basic bioinformatics - Experience presenting scientific findings or contributing to multidisciplinary research

What do we offer? A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of

the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better

health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education

in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective

agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site. Location The

position is located within the Department of Medicine, Solna. The department consists of ten divisions and each division consists

of several research groups. The position is located within the research group of Sophie Bensing within the Division of

Experimental Endocrinology, with research activities connected to immune regulation, single-cell genomics, endocrinology and

autoimmune disease. --[Video about Karolinska Institutet, how we all work towards "a better health for all"] -- Application KI

applies individual and differentiated salary setting in accordance with our collective agreements RALS and RALS-T. The application

is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal

letter. Instead, you will answer some questions about why you are applying for the job in the application form. Want to make a

difference? Join us and contribute to better health for all